[2014-02-05 21:48:17] Beginning TopHat run (v2.0.9) ----------------------------------------------- [2014-02-05 21:48:17] Checking for Bowtie Bowtie version: 2.1.0.0 [2014-02-05 21:48:17] Checking for Samtools Samtools version: 0.1.18.0 [2014-02-05 21:48:17] Checking for Bowtie index files (genome).. [2014-02-05 21:48:17] Checking for reference FASTA file [2014-02-05 21:48:17] Generating SAM header for Crassostrea_gigas.GCA_000297895.1.21.dna_rm.toplevel format: fastq quality scale: phred33 (default) [2014-02-05 21:48:20] Reading known junctions from GTF file [2014-02-05 21:48:22] Preparing reads left reads: min. length=49, max. length=49, 26647738 kept reads (618 discarded) [2014-02-05 21:54:17] Building transcriptome data files.. [2014-02-05 21:54:21] Building Bowtie index from Crassostrea_gigas.GCA_000297895.1.21.fa [2014-02-05 21:55:04] Mapping left_kept_reads to transcriptome Crassostrea_gigas.GCA_000297895.1.21 with Bowtie2 /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-02-05 22:01:09] Resuming TopHat pipeline with unmapped reads [2014-02-05 22:01:09] Mapping left_kept_reads.m2g_um to genome Crassostrea_gigas.GCA_000297895.1.21.dna_rm.toplevel with Bowtie2 /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx) [2014-02-05 22:09:52] Mapping left_kept_reads.m2g_um_seg1 to genome Crassostrea_gigas.GCA_000297895.1.21.dna_rm.toplevel with Bowtie2 (1/2) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-02-05 22:12:27] Mapping left_kept_reads.m2g_um_seg2 to genome Crassostrea_gigas.GCA_000297895.1.21.dna_rm.toplevel with Bowtie2 (2/2) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-02-05 22:16:25] Searching for junctions via segment mapping [2014-02-06 00:09:40] Retrieving sequences for splices [2014-02-06 00:10:33] Indexing splices [2014-02-06 00:21:40] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-02-06 00:27:41] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-02-06 00:34:57] Joining segment hits [2014-02-06 00:36:41] Reporting output tracks ----------------------------------------------- [2014-02-06 00:46:08] A summary of the alignment counts can be found in ./tophat_out/align_summary.txt [2014-02-06 00:46:08] Run complete: 02:57:50 elapsed