Oly Transcript Primers

Still loading...

	A	B	C	D	E	F	G	H	I	J	O	P	Q	R	S	T	U	V	W	X	Y	Z	AA	AB	AC	AD	AE	AF	AG	AH
1	Number	Accension#	E-Value	Function	SHORT Name	FWD	REV	Size
2	comp9638_c0_seq1	Q9Y547	6.00E-39	Heat shock protein beta-11 (Hspb11) (Placental protein 25) (PP25)	(Hspb11)	ATGTTTCCTGGTCTCCGTCA	CATCAACGCCAGGGGAACTT	139
3	comp9524_c1_seq1	O42222	2.00E-28	Growth/differentiation factor 8 (GDF-8) (Myostatin) (Myostatin-1) (zfMSTN-1) (Myostatin-B)	(GDF-8)	CCGTGGATGTCGCAGAAAGA	CTGCTTTCTCCGTCCCCTTT	172
4	comp9280_c0_seq1	Q96MM6	#######	Heat shock 70 kDa protein 12B	HSP70b	AAGTACCTTGGGGAGCTTGC	TCCACAGACTTTCCTCCCCA	155
5	comp8243_c0_seq1	Q90593	0	78 kDa glucose-regulated protein (GRP-78) (Heat shock 70 kDa protein 5) (Immunoglobulin heavy chain-binding protein) (BiP)	(GRP-78)	GAGAAACCACGCAGGGAGAA	CATCAGCATCGAAGGCAACG	227
6	comp7220_c0_seq2	Q6DC04	0	Histone-arginine methyltransferase CARM1 (EC 2.1.1.-) (EC 2.1.1.125) (Coactivator-associated arginine methyltransferase 1) (Protein arginine N-methyltransferase 4)	CARM1	TGGTTATCAACAGCCCCGAC	GTTGTTGACCCCAGGAGGAG	261
7	comp7183_c0_seq1	P12643	2.00E-93	Bone morphogenetic protein 2 (BMP-2) (Bone morphogenetic protein 2A) (BMP-2A)	(BMP-2)	TGAAGGAACGACCAAAGCCA	TCCGGTTGAAGAACCTCGTG	373
8	comp6939_c0_seq1	P32240	1.00E-50	Prostaglandin E2 receptor EP4 subtype (PGE receptor EP4 subtype) (PGE2 receptor EP4 subtype) (Prostanoid EP4 receptor)	PGE/EP4	ACAGCGACGGACGATTTTCT	ATGGCAGACGTTACCCAACA	303
9	comp25313_c0_seq1	Q60803	#######	TNF receptor-associated factor 3 (EC 6.3.2.-) (CD40 receptor-associated factor 1) (CRAF1) (TRAFAMN)	CRAF1	AGCAGGGCATCAAACTCTCC	ACAAGTCGCACTGGCTACAA	119
10	comp24195_c0_seq1	Q91974	5.00E-36	NF-kappa-B inhibitor alpha (I-kappa-B-alpha) (IkB-alpha) (IkappaBalpha) (REL-associated protein pp40)	NFKBina	GATGGCGGTGCATGTGTTAG	CGAGGAGAACCTTGTGCAGT	227
11	comp24065_c0_seq1	O75594	2.00E-42	Peptidoglycan recognition protein 1 (Peptidoglycan recognition protein short) (PGRP-S)	(PGRP-S)	GAGACTTCACCTCGCACCAA	AACTGGTTTGCCCGACATCA	135
12	comp23747_c0_seq1	Q9DD78	8.00E-29	Toll-like receptor 2 type-1	TLR2.1	ACAAAGATTCCACCCGGCAA	ACACCAACGACAGGAAGTGG	109
13	comp23265_c1_seq1	Q8AVB2	5.00E-39	Growth/differentiation factor 8 (GDF-8) (Myostatin)	(GDF-8)b	AACTGATTCTGCTCGTCGCA	TGTTCTTCCACCCACCACTG	126
14	comp22403_c0_seq1	Q8K0U4	#######	Heat shock 70 kDa protein 12A	HSP70c	AGGAAAGGTCGGGAGAGGAA	ACCTCGGACTTTGGACGAAC	182
15	comp22144_c0_seq1	Q8C0D7	6.00E-42	Inhibitor of growth protein 4 (p29ING4)	p29ING4	TACCTTTGGGCTTCACCGTC	GTCCATCACACACCCCTCAG	173
16	comp20292_c2_seq1	Q3ZBF8	4.00E-25	Ceramide synthase 2 (CerS2) (LAG1 longevity assurance homolog 2)	CerS2	TTGTCGGTCTCCTCCTGCTA	CCGTCTTCTGAGCCATCGTT	227
17	comp19002_c0_seq1	Q9WV18	1.00E-27	Gamma-aminobutyric acid type B receptor subunit 1 (GABA-B receptor 1) (GABA-B-R1) (GABA-BR1) (GABABR1) (Gb1)	GABABR1	CCGAGGAGGACACGAAACTC	CGGACAGGTTCTGGATTCCG	361
18	comp16752_c2_seq1	P17066	2.00E-27	Heat shock 70 kDa protein 6 (Heat shock 70 kDa protein B')	HSP70d	TTTGTCTCACCGGCTTTGTG	GACATGAGACCAAAGACGCC	118
19	comp16251_c0_seq1	Q9W6N4	#######	Thyroid hormone receptor alpha (Nuclear receptor subfamily 1 group A member 1)	THRa	GACACTATCCTCACTCGGCG	GGGTGCCGAGTAAACAAGGA	373
20	comp10930_c0_seq1	P80957	1.00E-23	Big defensin	Defensin	TCTAGCGGAGTTTGTTGGGG	ATGGCTGTCGGAGGAGGATT	118
21	comp10127_c0_seq1	P62994	1.00E-83	Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)	GRB2	AACTTTGTCCACCCAGACGG	CCAGTTGCAGTCCACTTCCT	351
22	comp19571_c0_seq1	Q6P823	2.00E-90	Histone H3.3	H3.3	CACGCTCTCCTCGAATCCTC	AAGTTGCCTTTCCAGCGTCT	214
23	comp25000_c0_seq1	P08991	5.00E-64	Histone H2A.V (H2A.F/Z) (Fragment)	H2A.V	TGCTTTCTGTGTGCCCTTCT	TATCACACCCCGTCACTTGC	139
24	comp23253_c0_seq1	P02270	2.00E-49	Histone H2A	H2A	GCTGGGGTTTTTCTGGGTCT	GGAACTACGCCGAGAGAGTG	266
25
26	comp25040_c1_seq1	A5D989	8.00E-32	Elongation factor 1-delta (EF-1-delta)	EF1d	GAACTGCCCACTGATTTGCC	TGTGGGGTGAAACACGTTGA	220
27	comp8371_c0_seq1	B3MK91	0	"Elongation factor G, mitochondrial (EF-Gmt) (Elongation factor G 1, mitochondrial) (mEF-G 1) (Elongation factor G1) (Protein iconoclast)"	EFG	ACCCAAGGACAGGGAGAGTT	TGTGTCCCCTTGTTGCTCTC	113
28	comp22728_c1_seq1	P53498	2.00E-92	Actin	Actin	GTTCTCCTGTCGGGTGGAAG	CACCATGAAACGCCGACTTG	123
29	comp2907_c0_seq1	Q6BMK0	1.00E-77	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12)	GAPDH	TGCTCCTTGCATTTCCGTCA	CGCTCCTTCCAAGTCTCCAG	221
30
31	comp18697_c0_seq1	Q5REJ1	9.00E-55	"28S ribosomal protein S5, mitochondrial (MRP-S5) (S5mt)"
32	comp19168_c0_seq1	P46782	3.00E-131	"40S ribosomal protein S5 [Cleaved into: 40S ribosomal protein S5, N-terminally processed]"
33	comp7293_c0_seq1	O61231	2.00E-128	60S ribosomal protein L10 (QM protein homolog) (dQM)
34
35
36
37
38
39
40
41
42
43
44
45	Rank	Primer Name			Gene	Primer	FWD	REV	Size	Melt temp
46	1	Flk_TLR		20	Toll-like receptor 2 type-1	TLR2.1	GCAATAGCTTGTCACCGCC	TCTAGTATGCGCTTCGTTTGC	425	59
47	2	Flk_CRAF		21	TNF receptor-associated factor 3 (EC 6.3.2.-) (CD40 receptor-associated factor 1) (CRAF1) (TRAFAMN)	CRAF 1	GGACATCCAGTGGCAACATTC	CCAGGACATTAGGCTTGCTGA	423	60
48	3	Flk_PGRP		20-25	Peptidoglycan recognition protein 1 (Peptidoglycan recognition protein short) (PGRP-S)	PGRP-S	AGCTGGTGCAGTCCTATCAG	TGTGTATGAAAAGTAATGAAGAGCA	450	59-57
49	4	Flk_CARM		21	Histone-arginine methyltransferase CARM1 (EC 2.1.1.-) (EC 2.1.1.125) (Coactivator-associated arginine methyltransferase 1) (Protein arginine N-methyltransferase 4)	CARM1	TTCACACAGCCCATTGTGGAT	TGGGATGGGTCAGATAAACCT	638	60-58
50	5	Flk_BMP2		18-21	Bone morphogenetic protein 2 (BMP-2) (Bone morphogenetic protein 2A) (BMP-2A)	BMP2	GGCTGGCTGGATCGTCAT	ATGGAGTCTGTGGACGGTTTG	676	60
51	6	Flk_HSPb11		22-20	Heat shock protein beta-11 (Hspb11) (Placental protein 25) (PP25)	HSPb11	AGAATTGTCTGTGGAATCGAGC	ATCAACGCCAGGGGAACTTG	323	59-61
52	7	Flk_PGE/EP4		22-23	Prostaglandin E2 receptor EP4 subtype (PGE receptor EP4 subtype) (PGE2 receptor EP4 subtype) (Prostanoid EP4 receptor)	PGE/EP4	GCTCAACGAATTGCTCTACTCC	TCCGTCTGCTTTTTAGAATGGTA	654	59-58
53	8	Flk_GABABR		20	Gamma-aminobutyric acid type B receptor subunit 1 (GABA-B receptor 1) (GABA-B-R1) (GABA-BR1) (GABABR1) (Gb1)	GABABR	GAGGAGGACACGAAACTCCG	TGCACCACACTCCTGATGAC	558	60
54	9	Flk_GRB2		23-20	Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)	GRB2	TCAGAACTGGTTCAAAGCTGAGT	ACTGCGCTGACATACTGGAC	695	60
55	10	Flk_H3.3		23-21	Histone H3.3	H3.3	CCAATGACAAATGAGCCACACAA	TCGTACAAAGCAAACTGCACG	552	60
56	11	Flk_H2A.V		20	Histone H2A.V (H2A.F/Z) (Fragment)	H2A.V	GCGATGGAGTTGATGAGGTG	CAAGGCAGTTTCTCGTTCGG	487	59
57	12	Flk_H2A		20-21	Histone H2A	H2A	TGCTGGGGTTTTTCTGGGTC	TCAGGACGTGGTAAAGGAGGA	373	60
58	13	Flk_p29ING		20	Inhibitor of growth protein 4 (p29ING4)	p29ING	GTGGACACACATGCACTCCT	AAGCAGACTCAGATTCAGGC	514	60-58
59	14	Flk_HSP70		20-19	Heat Shock Protein 70 kDa	HSP70	GACCCGGATGTCCAGAGGAA	TCAGGACGGCTTGTGAACG	1068	60
60	15	Flk_Actin		20	Actin	Actin	TCGAGGGAGGAAGAGGAAGC	AACTGGGACGACATGGAGAA	479	59-61
61	2x Apex Red	12.5	125		95 C	30 sec		Agarose	1.3 g
62	Forward Primer (10uM)	0.5	5		55 C	30 sec		EtBr	10 ul			TLR2.1									CRAF 1									Empty
63	Reverse Primer (10uM)	0.5	5		72 C	30 sec					Ladder	NTC	NT1	HT1	ST1	NC1	HC1	SC1	NTC	Ladder	NTC	NT1	HT1	ST1	NC1	HC1	SC1	NTC	Ladder	Empty
64	H20	10.5	105		repeat steps 2-4 40 times
65	Template	1			72 C	3 min
66		25			4 C	Hold
67
68												PGRP-S								CARM								BMP2
69											Ladder	NT1	HT1	ST1	NC1	HC1	SC1	NTC	Ladder	NT1	HT1	ST1	NC1	HC1	SC1	NTC	Ladder	NT1	HT1	ST1
70		Ladder	NT1	HT1	ST1	NC1	HC1	SC1	NTC
71
72											BMP2					HSPb11								PGE/EP4
73											NC1	HC1	SC1	NTC	Ladder	NT1	HT1	ST1	NC1	HC1	SC1	NTC	Ladder	NT1	HT1	ST1	NC1	HC1	SC1	NTC
74
75
76
77
78
79												Actin								HSP70
80											Ladder	HC1	NC1	SC1	HT1	NT1	ST1	NTC	Ladder	HC1	NC1	SC1	HT1	NT1	ST1	NTC	Empty	Empty	Empty	Empty
81
82
83												PGEEP4								HSPb11
84											Ladder	HC1	NC1	SC1	HT1	NT1	ST1	NTC	Ladder	HC1	NC1	SC1	HT1	NT1	ST1	NTC	Empty	Empty	Empty	Empty
85
86
87
88												BMP2
89											Ladder	HC1	NC1	SC1	HT1	NT1	ST1	NTC	Empty	Empty	Empty	Empty	Empty	Empty	Empty	Empty	Empty	Empty	Empty	Empty
90
91
92
93
94
95
96
97
98
99
100