FastQCFastQC Report
Mon 13 Apr 2015
2112_lane1_ACAGTG_L001_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_ACAGTG_L001_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327210
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC26027019.610310350283676TruSeq Adapter, Index 5 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC200961.5141537511019356TruSeq Adapter, Index 5 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT128470.9679704040807409TruSeq Adapter, Index 5 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC106440.8019831074208301TruSeq Adapter, Index 5 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC96440.7266370807935444TruSeq Adapter, Index 5 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC51740.38984034176957677TruSeq Adapter, Index 5 (98% over 50bp)
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC32980.2484911958167886No Hit
CGCAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT31380.2364358315564229TruSeq Adapter, Index 5 (98% over 50bp)
CGTAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT30120.22694223220138485TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCGATCTCGTATGC26540.19996835466881657TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGACTCGTATGCC18670.1406710317131426TruSeq Adapter, Index 5 (97% over 40bp)
AACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA17930.13509542574272346No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGAGATCTCGTATGC17500.13185554659775017TruSeq Adapter, Index 5 (98% over 50bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC16860.12703340089360388TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGGGATCTCGTATGC15060.11347111610069242TruSeq Adapter, Index 5 (98% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCT14620.11015589092909185TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAG25050.089.1225363
ACCGGAA24900.088.3240662
GACCGGA23200.087.425331
TCTGAAG20400.084.0571063
CTGAAGA22750.082.0525744
GAAGAGC470650.079.5129246
TTGGAAG15100.079.272223
TGAAGAG23550.079.262235
CATCGGA13850.079.224521
GAGCACA472200.079.111119
AGAGCAC473250.078.955658
AAGAGCA477500.078.322537
ACGGAAG14600.078.082883
GGAAGAG458400.077.524295
CGGAAGA444200.076.8938754
TCGGAAG396650.076.067723
TGGAAGA18100.075.31554
ATTGGAA16050.074.8760452
ATCGGAA399900.074.368622
TCTCTGA10000.073.625641
Produced by FastQC (version 0.11.2)