FastQCFastQC Report
Mon 13 Apr 2015
2112_lane1_ATCACG_L001_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_ATCACG_L001_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC15224309.5151875TruSeq Adapter, Index 1 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC986410.61650625TruSeq Adapter, Index 1 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT976470.61029375TruSeq Adapter, Index 1 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC682490.42655625TruSeq Adapter, Index 1 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC419830.26239375TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGATCTCGTATGC189210.11825625000000001TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA135950.081.6981661
CCGGAAG144450.080.1749653
ACCGGAA150350.076.8425452
GAGCACA2768550.072.7699
AGAGCAC2800200.071.967098
AAGAGCA2851050.070.755157
GAAGAGC2853300.070.734316
GGAAGAG2913700.068.872545
CGGAAGA2839150.067.796514
TCGGAAG2582600.067.182423
ATCGGAA2648200.065.499562
GATCGGA2666650.063.8482551
ACGGAAG81800.062.0793233
AATCATA1593350.061.505715
GAACGGA81150.060.00431
ATCATAA1673400.059.5109256
CATAAAC1670900.059.1025168
GATTGGA133550.059.0843961
TCATAAA1678750.058.9053737
TAAATCA1521250.058.206023
Produced by FastQC (version 0.11.2)