FastQCFastQC Report
Mon 13 Apr 2015
2112_lane1_GCCAAT_L001_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_GCCAAT_L001_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8792684
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC90959510.344907197847665TruSeq Adapter, Index 6 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT628340.7146168337222173TruSeq Adapter, Index 6 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC589880.6708759236656292TruSeq Adapter, Index 6 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC399710.4545938418803633TruSeq Adapter, Index 6 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC248010.28206404324322354TruSeq Adapter, Index 6 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC182350.20738832420225725TruSeq Adapter, Index 6 (98% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCT136170.15486738747804424TruSeq Adapter, Index 6 (100% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC119420.13581745915126714TruSeq Adapter, Index 6 (98% over 50bp)
CGCAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT93610.10646350989072279TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAAATATCTCGTATGC89490.10177779617691254TruSeq Adapter, Index 6 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA76150.084.7816541
CCGGAAG80950.084.739883
ACCGGAA84050.081.502792
GATTGGA59900.071.8544541
GAGCACA1660300.071.617169
AGAGCAC1673750.071.0984048
CATCGGA30850.070.990141
ACGGAAG43750.070.9043053
GAAGAGC1690250.070.5287866
AAGAGCA1705300.069.881287
GGAAGAG1694650.069.124515
GAACGGA42100.068.946371
CGGAAGA1644100.068.337014
TCGGAAG1484500.068.129143
TTGGAAG65800.067.069853
AATCATA1243600.066.93465
ATCGGAA1518800.066.44782
ATTGGAA67150.065.864072
TAAATCA1134900.065.4744953
TCATAAA1293250.064.955417
Produced by FastQC (version 0.11.2)