FastQCFastQC Report
Mon 13 Apr 2015
2212_lane2_CTTGTA_L002_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2212_lane2_CTTGTA_L002_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC178440.11152499999999999TruSeq Adapter, Index 12 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAAAT1314500.072.333951
ATAAATC1377350.069.814092
TAAATCA1500800.065.028153
TCATAAA1680100.058.8678747
AATCATA1708150.057.781625
ATCATAA1843600.054.1340686
AAATCAT1790150.054.1273544
CATAAAC2128200.046.6627278
ATAAACA2288250.043.2660989
GTCCCCA30500.032.407151
ACAAACG1760100.029.34278312-13
AGTAAAT29100.027.9241851
AAACGGG1851800.027.4922414-15
CAAACGG1824600.026.91031614-15
ACGGGCA2007900.025.4531216-17
GGGCAAC2070250.024.43319118-19
AACGGGC1976800.024.39756416-17
GTAAATC34800.024.0275962
TGATACG26850.023.8805433
CCCCGCA53250.023.6485021
Produced by FastQC (version 0.11.2)