FastQCFastQC Report
Mon 13 Apr 2015
2212_lane2_CTTGTA_L002_R1_004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2212_lane2_CTTGTA_L002_R1_004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10634369
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC118650.11157220517738287TruSeq Adapter, Index 12 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAAAT862150.072.225061
ATAAATC908500.069.971882
TAAATCA988700.065.390043
TCATAAA1108050.059.81967
AATCATA1132700.058.207795
ATCATAA1224250.054.661666
AAATCAT1189900.054.4889144
CATAAAC1397750.047.614978
ATAAACA1502950.044.256859
GTCCCCA20000.030.1630211
ACAAACG1172150.029.3824912-13
TGATACG17350.028.1962873
AAACGGG1229150.027.56203714-15
CAAACGG1212650.026.91678814-15
ACGGGCA1333000.025.44504516-17
AACGGGC1309800.024.4054216-17
GGGCAAC1381350.024.30502918-19
GTCCCCC15650.023.3710121
CGGGCAA1374550.023.13645618-19
CCCCGCA38850.022.9862421
Produced by FastQC (version 0.11.2)