FastQCFastQC Report
Mon 13 Apr 2015
2212_lane2_GCCAAT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2212_lane2_GCCAAT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAATC4443800.079.229982
AATAAAT4547100.076.660181
TAAATCA4686350.075.7207953
AATCATA5066050.071.2315755
TCATAAA5098300.070.873227
AAATCAT5083500.069.8387154
ATCATAA5272500.069.069456
CATAAAC5557250.064.940628
ATAAACA6230450.057.9390349
ATGAATC24700.055.405222
GTCCCCA19300.044.8260881
TGCCCGT216200.043.5997281
GTCCCCC11350.042.7189981
AGAAATC45650.041.5324252
CCCCGTT18150.039.0235141
ACAAACG4937100.037.7043712-13
GAATCAT37100.037.384514
TTGCCCG122300.036.4580231
AAACGGG5050300.036.43036314-15
GATAAAT49050.036.3420141
Produced by FastQC (version 0.11.2)