FastQCFastQC Report
Mon 13 Apr 2015
2212_lane2_GCCAAT_L002_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2212_lane2_GCCAAT_L002_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAATC4468700.079.114892
AATAAAT4547100.076.718721
TAAATCA4712050.075.645913
AATCATA5101900.071.4314965
TCATAAA5139400.071.191277
AAATCAT5110150.069.961944
ATCATAA5316050.069.293766
CATAAAC5602850.065.204228
ATAAACA6296800.058.019789
ATGAATC28850.055.9954722
GATAAAT57250.045.9074861
AGAAATC51500.045.391832
GTAAATC24200.045.3540882
GTGCAAC16900.044.9834862
TGCCCGT208650.044.2804221
CGTGCAA16500.043.781791
GAATCAT38900.043.223654
CCCCGTT20450.041.3676221
GTCCCCC11300.041.2174871
GTCCCCA18200.039.9534151
Produced by FastQC (version 0.11.2)