FastQCFastQC Report
Mon 13 Apr 2015
2212_lane2_GCCAAT_L002_R1_003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2212_lane2_GCCAAT_L002_R1_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAATC4437900.079.0570142
AATAAAT4548850.076.312071
TAAATCA4692250.075.685283
AATCATA5025400.071.082165
TCATAAA5073300.070.82677
AAATCAT5043750.069.5443954
ATCATAA5246200.068.973186
GTAAATC44900.067.71112
AGTAAAT45350.066.7349851
CATAAAC5528000.064.873768
ATAAACA6179600.057.813429
AAATCAG51550.045.976624
GTCCCCA18950.045.6303251
ATGAATC21900.045.1175462
GTCCCCC10400.043.3991971
AGAAATC47350.043.2397842
TGCCCGT205050.042.12361
AATCAGA60400.039.2391245
GATAAAT52050.038.7935561
TTGCCCG118600.037.6960951
Produced by FastQC (version 0.11.2)