FastQCFastQC Report
Mon 13 Apr 2015
2212_lane2_GCCAAT_L002_R1_004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2212_lane2_GCCAAT_L002_R1_004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAATC4518450.079.4681242
AATAAAT4595200.076.543371
TAAATCA4784200.076.040543
TCATAAA5241500.071.779387
AATCATA5179600.071.7770545
AAATCAT5203300.070.1885764
ATCATAA5414800.069.889976
CATAAAC5706850.065.8439258
ATAAACA6403900.058.672519
AGAAATC55900.053.7860682
GATAAAT64150.049.8323361
GTAAATC28500.048.498242
GTGCAAC18900.048.0009082
GTCCCCA18200.047.2389261
AGTAAAT28050.046.7378851
CGTGCAA18950.045.619971
CGCTTGT19550.043.245843
AGTCATA16100.043.0716325
AAGAAAT69000.041.6484761
ATGAATC18950.041.6079942
Produced by FastQC (version 0.11.2)