FastQCFastQC Report
Mon 13 Apr 2015
2212_lane2_GCCAAT_L002_R1_005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2212_lane2_GCCAAT_L002_R1_005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAATC4451550.079.195672
AATAAAT4517050.076.378281
TAAATCA4729900.075.94733
AATCATA5135100.071.569945
TCATAAA5193700.071.435027
AAATCAT5133400.070.1265954
ATCATAA5372000.069.607776
CATAAAC5651000.065.492868
ATAAACA6327200.058.512289
GTAAATC34400.057.440842
AGTAAAT36500.052.575721
GTCCCCA18800.048.510991
GATAAAT60700.045.5439871
CGCTTGT20900.043.6328623
AGAAATC48450.042.744342
TGCCCGT194200.041.7279051
ATGAATC17550.041.4095232
CCGCTTG20800.041.3333972
CCCCGTT20750.040.9762041
GGCCCGT12600.039.583621
Produced by FastQC (version 0.11.2)