FastQCFastQC Report
Mon 13 Apr 2015
2212_lane2_GCCAAT_L002_R1_006.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2212_lane2_GCCAAT_L002_R1_006.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255678
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTGT156.613179E-494.99571
CATACGG156.613179E-494.99573
ATAAATC73400.080.3063052
TAAATCA79300.077.625743
AATAAAT72800.076.923031
TCATAAA89650.074.000587
CCGCTTG452.41198E-973.8855442
ATCATAA91550.072.8278966
AATCATA85800.072.520035
AAATCAT85750.071.565274
CCCGCTA200.002072598871.246772
AGCCCAC353.410336E-667.8540651
CGATTGT700.067.8540653
CATAAAC97900.067.813088
CTTGTTT505.5497367E-966.496995
CGCTTGT505.5497367E-966.496993
CCGATTG659.094947E-1265.766252
CCCGATT659.094947E-1265.766251
CCCCGTT301.2995896E-463.3304671
CCGGTTG301.2995896E-463.3304672
Produced by FastQC (version 0.11.2)