FastQCFastQC Report
Sat 14 Mar 2015
2112_lane1_NoIndex_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_NoIndex_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC5004613.12788125TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3848432.40526875TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC3059941.9124625TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2919931.82495625TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2424401.51525TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2095111.30944375TruSeq Adapter, Index 6 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT317800.19862500000000002TruSeq Adapter, Index 7 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT240090.15005625TruSeq Adapter, Index 4 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT220190.13761875TruSeq Adapter, Index 5 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT202770.12673125TruSeq Adapter, Index 1 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGT165610.10350625TruSeq Adapter, Index 3 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT164340.1027125TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA142400.081.3737261
CCGGAAG142450.081.166743
ACCGGAA152200.077.177312
GAGCACA3718200.071.7571269
AGAGCAC3757900.071.002258
AAGAGCA3823900.069.9111257
GAAGAGC3844450.069.630896
TCGGAAG3631900.068.527943
GGAAGAG3923000.068.4708565
CGGAAGA3880500.067.673974
ATCGGAA3717700.067.129672
GATCGGA3784800.065.83111
GATTGGA101150.059.8637661
AATCATA1236700.058.4776425
ATTGGAA106550.057.4405022
TTGGAAG108500.056.828343
TAAATCA1227300.055.7083853
TCATAAA1312200.054.901347
TGGAAGA121900.054.825754
ATCATAA1326150.054.595696
Produced by FastQC (version 0.11.2)