FastQCFastQC Report
Mon 13 Apr 2015
2212_lane2_CTTGTA_L002_R1_003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2212_lane2_CTTGTA_L002_R1_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC163570.10223125TruSeq Adapter, Index 12 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAAAT1328150.072.4921951
ATAAATC1399050.070.2604752
TAAATCA1517900.065.3518753
TCATAAA1713050.059.8247537
AATCATA1734300.058.5166175
ATCATAA1871900.055.1438336
AAATCAT1820200.054.6886754
CATAAAC2164650.047.4688878
ATAAACA2342500.043.8953179
ACAAACG1807100.029.47825812-13
GTCCCCA31800.028.6800651
AAACGGG1900700.027.7054914-15
TGATACG27350.027.2652233
CAAACGG1869550.027.1165914-15
ACGGGCA2056600.025.61221116-17
AACGGGC2024700.024.58710316-17
GGGCAAC2119800.024.55998618-19
GTCCCCC22900.024.0618271
GATACGG31150.023.7866484
ATACGGC31500.023.3712025
Produced by FastQC (version 0.11.2)