Today I continued my assessment of DNA quality for P. astreoides samples extracted earlier this winter by running them on a gel (using this protocol). Here is the gel:
Again, quite a range of quality. I now have looked at nearly all samples and here is my assessment (this data can also be found in this spreadsheet):
| Sample # | Qubit conc. (ng/ul) | Sample volume | Total DNA quantity (ng) | run on gel? | high MW DNA present? | relative quality score (1 = low) |
| past_87 | 45.6 | 50 | 2280 | y | yes – very good | 5 |
| past_89 | 48 | 50 | 2400 | y | some – w/ smearing | 3 |
| past_85 | 143 | 50 | 7150 | y | yes – very good | 5 |
| past_88b | 121 | 50 | 6050 | y | yes – very good | 5 |
| past_84 | 188 | 50 | 9400 | y | some – w/ smearing | 3 |
| past_91 | 194 | 50 | 9700 | y | yes – ok | 4 |
| past_88a | 182 | 50 | 9100 | y | yes – ok | 4 |
| past_86 | 234 | 50 | 11700 | y | yes – ok | 4 |
| past_90 | 218 | 50 | 10900 | y | yes – very good | 5 |
| past_83 | 194 | 50 | 9700 | y | little – poor | 1 |
| past_15 | 5.92 | 200 | 1184 | y | yes – ok | 4 |
| past_16 | 3.46 | 200 | 692 | n | nd | nd |
| past_96 | 6.82 | 200 | 1364 | y | some – w/ smearing | 3 |
| past_19 | 5.4 | 200 | 1080 | y | some – mostly low MW | 2 |
| Past_14 | 3.92 | 200 | 784 | n | nd | nd |
| past_82 | 7.38 | 200 | 1476 | y | yes – very good | 5 |
| past_98 | 7.92 | 200 | 1584 | y | yes – very good | 5 |
| past_13 | 10.4 | 200 | 2080 | y | yes – ok | 4 |
| past_12 | 3.1 | 200 | 620 | n | nd | nd |
| past_18 | 5.88 | 200 | 1176 | y | some – w/ smearing | 3 |
| past_99 | 5.16 | 200 | 1032 | y | some – w/ smearing | 3 |
| past_17 | 10 | 200 | 2000 | y | yes – ok | 4 |
| past_95 | 5.88 | 200 | 1176 | y | some – mostly low MW | 2 |
| past_11 | 10.4 | 200 | 2080 | y | yes – very good | 5 |
| past_80 | 10.8 | 200 | 2160 | y | yes – ok | 4 |
| past_81 | 8.82 | 200 | 1764 | y | some – w/ smearing | 3 |
| past_100 | 2.28 | 200 | 456 | n | nd | nd |
| past_1 | 11.3 | 200 | 2260 | y | yes – very good | 5 |
| past_10 | 20 | 200 | 4000 | y | yes – very good | 5 |
| past_97 | too low | 200 | nd | n | nd | nd |
| past_10-2 | nd | 200 | nd | n | nd | nd |
| past_1-2 | nd | 200 | nd | n | nd | nd |
| past_97-2 | nd | 200 | nd | n | nd | nd |
| past_100-2 | nd | 200 | nd | n | nd | nd |
| past_w11 | 35.2 | 50 | 1760 | y | some – mostly low MW | 2 |
| past_3 | 36.6 | 50 | 1830 | y | some – w/ smearing | 3 |
| past_2 | 99.4 | 50 | 4970 | y | yes – ok | 4 |
| past_w1 | 330 | 50 | 16500 | y | yes – ok | 4 |
| past_4 | 47.2 | 50 | 2360 | y | band too faint | |
| past_w3 | 172 | 50 | 8600 | y | yes – ok | 4 |
| past_5 | 77 | 50 | 3850 | y | yes – ok | 4 |
| past_6 | 66.2 | 50 | 3310 | y | some – w/ smearing | 3 |
| past_7 | 27.4 | 50 | 1370 | y | some – mostly low MW | 2 |
| past_9 | 244 | 50 | 12200 | y | some – w/ smearing | 3 |
| past_8 | 37.6 | 50 | 1880 | y | yes – very good | 5 |
| past_12 | 18.2 | 50 | 910 | y | yes – ok | 4 |
| ppor_2 | 91.4 | 50 | 4570 | y | yes – ok | 4 |
| ppor_6 | 60 | 50 | 3000 | y | yes – ok | 4 |
| ppor_10 | 122 | 50 | 6100 | y | yes – ok | 4 |
| ppor_3 | 142 | 50 | 7100 | y | yes – ok | 4 |
| ppor_7 | 132 | 50 | 6600 | y | yes – very good | 5 |
| ppor_8 | 102 | 50 | 5100 | y | yes – very good | 5 |
| ppor_4 | 69.8 | 50 | 3490 | y | yes – very good | 5 |
| ppor_9 | 114 | 50 | 5700 | n | nd | nd |