I did some further RNA extractions of Porites astreoides and Anthopleura elegantissima samples using the Qiagen RNeasy kit. Further information on these samples can be found in the previous post. I again used Qiashredder columns for sample homogenization, and use the Qubit RNA HS assay for quantification. Here are the results:
Organism | Sample | RNA ng/ul | Sample vol (ul) | Notes |
A. elegantissima | A4 483 | >100 | 40 | |
A. elegantissima | M4 414 | <20 | 40 | |
A. elegantissima | H1 400 | >100 | 40 | |
A. elegantissima | H6 120 | >100 | 40 | |
A. elegantissima | M3 461 | >100 | 40 | lowest amount of starting material |
A. elegantissima | A2 438 | >100 | 40 | |
P. astreoides | past7-cbc | 75 | 40 | |
P. astreoides | past7-home | 99 | 40 | |
P. astreoides | past14-cbc | >100 | 40 | |
P. astreoides | past14-home | >100 | 40 | |
P. astreoides | past5-cbc | 67 | 40 | |
P. astreoides | past5-home | >100 | 40 |
I may do some dilutions later.