RNA extraction – coral and anemone samples

I did some further RNA extractions of Porites astreoides and Anthopleura elegantissima samples using the Qiagen RNeasy kit. Further information on these samples can be found in the previous post. I again used Qiashredder columns for sample homogenization, and use the Qubit RNA HS assay for quantification. Here are the results:

Organism Sample RNA ng/ul Sample vol (ul) Notes
A. elegantissima A4 483 >100 40
A. elegantissima M4 414 <20 40
A. elegantissima H1 400 >100 40
A. elegantissima H6 120 >100 40
A. elegantissima M3 461 >100 40 lowest amount of starting material
A. elegantissima A2 438 >100 40
P. astreoides past7-cbc 75 40
P. astreoides past7-home 99 40
P. astreoides past14-cbc >100 40
P. astreoides past14-home >100 40
P. astreoides past5-cbc 67 40
P. astreoides past5-home >100 40

I may do some dilutions later.

Leave a Reply