Followed the rigorous protocol for Ambion’s Turbo DNA-free protocol for the following RNAs:
BB#11-20
DH#11-20
Used 10ug of RNA (200ng/uL) in 50uL as directed. Here are the calcs for FF and DH.
Followed standard protocol on DNased samples from 20090508:
BB#1-10
DH#1-10
The standard protocol should be fine for these samples, since the procedure worked on Friday for some of them.