Lane 1 – 15ng standard (5uL)
Lane 2 – 31ng standard (5uL)
Lane 3 – 63ng standard (5uL)
Lane 4 – Marker 2 (5uL)
Lane 5 – C. pugetti DNA (5uL: 4uL + 1uL 5x dye)
Lane 6 – Marker 3 (5uL)
Lane 7 – 125ng standard
Lane 8 – 250ng standard (5uL)
Lane 9 – 500ng standard (5uL)
Results: Looks great! Will run PCR using universal 16s primers for sequencing.