According to the Bio-Rad rep that analyzed the calibration test on 20090824, recalibration might be a good idea. As such, we’ll do that. Old calibration file was found and backed up prior to proceeding, as generation of a new calibration file would replace the old one.

Results: The Bio-Rad rep (Carl Fisher) has responded and said that the range of fluorescence is within the expected 2-fold difference and looks fine! Finally!