Primers – Lake Trout Primers for HRM

The two primer plates (LTP01F, LTP01R) were received today. Primers (supplied as 10 nmoles of each) will be reconstituted with 100uL of PCR H2O to make a Cf of 100uM. Forward and reverse primers will be combined in a new plate, in equal volumes, to give a Cf of 50uM. These combined primers will be used to test them on pooled lake trout DNA to identify functional primer pairs for use in HRM.

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