See the Next Gen Seq Library Database for more info. Processed the 4 samples (one set Ribominus only, one set Ribominus + PolyA enriched) according to the Agilent WTK. Briefly:
- Speedvac’d samples to dryness
- Resuspended RNA in 3uL H2O
- Adapter rxn. Used all 3uL of RNA (used only 1uL of RBC Ribo only sample due to high concentration)
- Ligation rxn
Incubated 16C for 16hrs.