qPCR was set up on these cDNAs using the following primers:
FP010108.p.cg.6 (“DJB12″, “DnaJ homolog subfamily B member 12″) – This was upregulated in DH SOLiD data.
AJ565670.p.cg.6 (“TOP1″, “DNA topoisomerase 1″) – This was upregulated in BB SOLiD data.
qPCR set up and plate layout can be found here.
Results:
qPCR Data File (Opticon): 20091229_164912.tad
Data workup: PROPS BB_DH Gene Expression Miner Workup