Ran colony PCR on various colonies produced from cloning on 20110411. All colonies were picked, re-streaked on Kan50 plate(s) and PCR’d. Master mix calcs are here. Cycling params:
- 95C – 10mins
40cycles of:
- 95C – 30s
- 55C – 30s
- 72C – 3mins
- 72C – 10mins
Results:
