Ran a qPCR on all cDNA samples. Created a standard curve to possibly allow for use of the BioRad software for gene expression analysis. Standard curve was created from pooled cDNA (1uL from each individual sample). Master mix calcs are here.
Results:
qPCR Data File (BioRad CFX96)
qPCR Report (PDF)
Standard curves aren’t that good. Will not use them. Will analyze data using PCR Miner.