Need more PCR product for sequencing. Repeated reaction from 20110825.
Results:
Lane 1 – Hypperladder I (Bioline)
Lane 2 – PCR 1 & 2
Lane 3 – PCR 3 & 4
Lane 4 – PCR 5 & 6
Lane 5 – Neg. Control
Bands from lanes 2 – 4 were excised and purified with Ultra-free DA columns (Millipore) and spec’d. Concentration was extremely low (3.5ng/uL) and too dilute for sequencing. Will EtOH precipitate.