Continued library prep from yesterday. Set up Library Amplification according to the protocol. The samples received the following Barcode Indices:

• HB2 – 1 (ATCACG)
• HB5 – 2 (CGATGT)
• HB16 – 3 (TTAGGC)
• HB30 – 4 (TGACCA)
• NB3 – 5 (ACAGTG)
• NB6 – 6 (GCCAAT)
• NB11 – 7 (CAGATC)
• NB21 – 12 (CTTGTA)
• 1A1 – 2 (CGATGT)
• 1A2 – 1 (ATCACG)
• 6A1 – 4 (TGACCA)
• 6A2 – 5 (ACAGTG)
• 103B1 – 6 (GCCAAT)
• 103B2 – 7 (CAGATC)
• 105A4 – 12 (CTTGTA)
• 105A5 – 11 (GGCTAC)

Due to differences in input DNA quantities, samples were run with different numbers of thermal cycles.

13 thermal cycles were run for the following samples:

• 1A1
• 105A4
• 105A5

22 thermal cycles were run for the following samples:

• HB2
• HB5
• HB16
• HB30
• NB3
• NB6
• NB11
• NB21
• 1A2
• 6A1
• 6A2
• 103B1
• 103B2

Samples were quantified with 1uL of each sample using the Quant-iT dsDNA BR Kit (Invitrogen). Used 5uL of each standard and standards were run in duplicate.

Results: