qPCR was set up on these cDNAs using the following primers:

EW778094 (“Ficolin 3″) –

AJ422120.p.cg.6 (“MDR49″, “Multidrug resistance protein homolog 49″) – This was upregulated in DH SOLiD data.

qPCR set up and plate layout can be found here.

Results:

qPCR Data File (Opticon): 20091224_092959.tad

Data workup: PROPS BB_DH Gene Expression Miner Workup

Preliminary results show that the EW778094 primer set looks bad; bad fluorescence profile and poor melt curves. Primers may require optimization.

qPCR was set up on these cDNAs using the following primers:

AM855874.p.cg.6 (“CP17A”, “Steroid 17-alpha-hydroxylase/17″) – This was upregulated in DH SOLiD data.

AM857078.p.cg.6 (“C1QT4″, Complement C1q tumor necrosis factor-related protein 4″) – This was upregulated in DH SOLiD data.

qPCR set up and plate layout can be found here.

Results:

qPCR Data File (Opticon): 20091224_125230.tad

Data workup: PROPS BB_DH Gene Expression Miner Workup