Tag Archives: QIAQuick Gel Extraction Kit

Gel Extraction – Oly RAD-Seq Prep Scale PCR

Extracted the PCR products from the gel slices from 20151113 using the QIAQuick Gel Extraction Kit (Qiagen) according to the manufacturer’s protocol. Substituted MiniElute columns so that I could elute with a smaller volume than what is used in the QIAQuick standard protocol.

Samples were eluted with 20μL of Buffer EB.

Will quantify these libraries via qPCR.