Tag Archives: rRNA

RNA Gel – V. tubiashii mRNA samples (from 20081224)

external image 20081229.png

Lane 1 – Empty
Lane 2 – Total RNA, Control
Lane 3 – mRNA, Control
Lane 4 – Total RNA, Vibrio+gigas
Lane 5 – mRNA, Vibrio+gigas

Results:

rRNA removal seems to have worked relatively well. Still some residual rRNA present in the mRNA samples. Will submit 200ng of the Vibrio+gigas mRNA to HTGU for Illumina sequencing.

rRNA Removal – V. tubiashii total RNA from yesterday

rRNA removal was continued from O/N precipitation. Processed the samples according to the Ambion MICROBExpress Kit protocol and resuspended final pellets in 25uL of The RNA Storage Solution. Samples were spec’d on the NanoDrop.

external image 20081224%20RNA%20SJW.PNG

Samples were stored @ -80C in Sam’s RNA Box #1.