Repeat of PCR from 20090403, but using AmpliTaq and 50C annealing temp. PCR set up is here.

Lane 1 – Hyperladder

Lane 2 – 19t

Lane 3 – 100bp ladder

Lane 4 – 13t

Lane 6 – 17t

Lane 8 – 1.5t

Lane 9 – Hyperladder

Lane 10 – 1.2t

Lane 12 – 11t

Lane 13 – 100bp ladder

Lane 14 – H5

Lane 16 – 12t

Lane 17 – 100bp Ladder

Lane 18 – H2O

Lane 19 – H2O

Lane 20 – Hyperladder

Results: Nothing amplified! Possibly due to age of polymerase (?); over a year old. Will wait to repeat for new primers to arrive (EukA/B).