Repeat of yesterday’s PCR, but with AmpliTaq, less gDNA and 50uL rxn volume. PCR set up is here.
Gel loaded and run by Steven. Not entirely sure of the loading order. However, it doesn’t really matter…
Results: Still absolutely nothing.
UPDATE: Noticed on 20090713 that the reactions didn’t have dNTPs. Probably explains why it didn’t work!
Repeat of PCR from 20090403, but using AmpliTaq and 50C annealing temp. PCR set up is here.
Lane 1 – Hyperladder
Lane 2 – 19t
Lane 3 – 100bp ladder
Lane 4 – 13t
Lane 6 – 17t
Lane 8 – 1.5t
Lane 9 – Hyperladder
Lane 10 – 1.2t
Lane 12 – 11t
Lane 13 – 100bp ladder
Lane 14 – H5
Lane 16 – 12t
Lane 17 – 100bp Ladder
Lane 18 – H2O
Lane 19 – H2O
Lane 20 – Hyperladder
Results: Nothing amplified! Possibly due to age of polymerase (?); over a year old. Will wait to repeat for new primers to arrive (EukA/B).
