Set up gDNA isolation from the following samples:
R51 01 – 20 (sample #11 was processed yesterday)
R37 01 – 03, 06 – 13, 15, 16
Samples were thawed from -80C. Tissue was removed from RNA Later (RNA Later gDNA isolation protocol; this protocol doesn’t indicate that anything needs to be done to the sample prior to gDNA isolation) and ~25mg was cut from each and placed in 0.5mL of DNAzol. 2.7uL of Proteinase K (Fermentas; 18.5mg/mL) was added to each tube to reach a final concentration of 100ug/mL. The digests were incubated @ RT O/N.