Prepared the RNA Pico 6000 ladder (Agilent) according to the manufacturer’s protocol:
- Denatured @ 70C for 2mins
- Immediately put on ice
- Added 90μL H2O
Made 2μL aliquots of the ladder in 0.5mL snap cap tubes. Samples were boxed, labeled, and stored @ -80C (Rack #9).