Re-quantified the libraries that were completed yesterday using the Qubit3.0 dsDNA HS (high sensitivity) assay because the library concentrations were too low for the normal broad range kit.
Results:
Qubit Quants and Library Normalization Calcs: 20151222_qubit_illumina_methylation_libraries
| SAMPLE | CONCENTRATION (ng/μL) |
| 1NF11 | 2.42 |
| 1NF15 | 1.88 |
| 1NF16 | 2.74 |
| 1NF17 | 2.54 |
| 2NF5 | 2.72 |
| 2NF6 | 2.44 |
| 2NF7 | 2.38 |
| 2NF8 | 1.88 |
| M2 | 2.18 |
| M3 | 2.56 |
| NF2_6 | 2.5 |
| NF_18 | 2.66 |
Things look pretty good. The TruSeq DNA Methylation Library Kit (Illumina) suggests that the libraries produced should end up with concentrations >3ng/μL, but we have plenty of DNA here to make a pool for running on the HiSeq2500.