Received a shipment of various MV oysters/cod samples from Scott Lindell at MBL. However, these were NOT shipped on dry ice! Samples were put @ -80C. Will be organized at a later date.
Tag Archives: Martha’s Vineyard
qPCR – MV hemocyte cDNA from 20090614
Set up qPCR with Cv_18s_F/R primers on the following samples that had previously come up negative in both reps using the diluted (1:20) cDNA that Mac had made specifically for the 18s runs:
3326A13
2100B07
3219A06
2100B15
3326A11
2100B12
2100A03
Plate layout/PCR set up is here. This is a second rep of these samples.
Results: Waters are clean. However, the following samples still are negative for an 18s amplicon:
qPCR – MV hemocyte cDNA from 20090614
Set up qPCR with Cv_18s_F/R primers on the following samples that had previously come up negative in both reps using the diluted (1:20) cDNA that Mac had made specifically for the 18s runs:
3326A13
2100B07
3219A06
2100B15
3326A11
2100B12
2100A03
Plate layout/PCR set up is here.
Results: Waters are clean. However, the following samples still are negative for an 18s amplicon:
qPCR – MV hemocyte cDNA from 20090614
Set up qPCR with Cv_CIAPIN_F/R primers. This is a second rep. Plate layout/PCR set up is here.
Results: Waters are clean. To be analyzed later with other genes that Mac has run.
qPCR – MV hemocyte cDNA from 20090614
Set up qPCR with Cv_CatY_F/R primers. This is a second rep. Plate layout/PCR set up is here.
Results: Waters are clean. To be analyzed later with other genes that Mac has run.
qPCR – MV hemocyte cDNA from 20090614
Set up qPCR with Cv_CatL_F/R primers. This is a second rep. Plate layout/PCR set up is here.
Results: Waters are clean. To be analyzed later with other genes that Mac has run.
qPCR – MV hemocyte cDNA from 20090614
Set up qPCR with Cv_TLR_”short”_F/R primers. This is a second rep. Plate layout/PCR set up is here.
Results: Waters are clean. To be analyzed later with other genes that Mac has run.
qPCRs – MV hemocyte cDNA from 20090614
qPCR – BgBL Primers
Set up qPCR with Cv_BgBL_F/R primers. This is a second rep. Plate layout/PCR set up is here.
Results: Waters are clean. To be analyzed later with other genes that Mac has run.
qPCR – HMG Primers
Set up qPCR with Cv_HMG_F/R primers. This is a second rep. Plate layout/PCR set up is here.
Results: Waters are clean. To be analyzed later with other genes that Mac has run.
qPCR – HSP70 Primers
Set up qPCR with Cv_HSP70_F/R primers. This is a second rep. Plate layout/PCR set up is here.
Results: Waters are clean. To be analyzed later with other genes that Mac has run.
qPCRs – MV hemocyte cDNA from 20090614
qPCR – CysB Primers
Set up qPCR with Cv_CysB_F/R. Plate layout/PCR set up is here.
Results: Waters are clean. To be analyzed later with other genes that Mac has run.
qPCR -BgBp Primers
Set up qPCR with Cv_BgBp_F/R primers. Plate layout/PCR set up is here.
Results: Waters are clean. To be analyzed later with other genes that Mac has run.
qPCR – MV hemocyte cDNA from 20090614
Set up qPCR with Cv_18s_F/R primers. Plate layout/PCR set up is here. This is a second rep. Used 1:20 cDNA plate made by Mac as template.
Results: Waters are clean. Still some samples that aren’t coming up. Will eventually run those samples using undiluted cDNA. To be analyzed later with other genes that Mac has run.