RAD-Seq Library Prep Reagents

A box with the above title was established in the -20C in FTR 209 containing the following:

  • Thermo Scientific AlfI: ER1801
  • NEB T4 DNA ligase, 50 μL: M0202S
  • NEB 10 mM ATP, 1 mL: P0756S
  • Promega dNTPs (10 mM each): U1511
  • NEB Q5 Taq Polymerase, 100 units: M0491S

Oligos (100μL each in TE pH=8.0; barcode sequences are in bold)

Adaptor 1
5ILL-NR: CTACACGACGCTCTTCCGATCTNR

Anti-ILL: AGATCGGAAGAGC(InvdT)

Adaptor 2
3ILL-NR: CAGACGTGTGCTCTTCCGATCTNR

ILL-Lib1: AATGATACGGCGACCACCGA

ILL-Lib2: CAAGCAGAAGACGGCATACGA

ILL-HT1: AATGATACGGCGACCACCGAGATCTACACATGCATACACTCTTTCCCTACACGACGCTCTTCCGATCT

ILL-HT2:AATGATACGGCGACCACCGAGATCTACACCGTACGACACTCTTTCCCTACACGACGCTCTTCCGATCT

ILL-BC1: CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC

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