A box with the above title was established in the -20C in FTR 209 containing the following:
- Thermo Scientific AlfI: ER1801
- NEB T4 DNA ligase, 50 μL: M0202S
- NEB 10 mM ATP, 1 mL: P0756S
- Promega dNTPs (10 mM each): U1511
- NEB Q5 Taq Polymerase, 100 units: M0491S
Oligos (100μL each in TE pH=8.0; barcode sequences are in bold)
Adaptor 1
5ILL-NR: CTACACGACGCTCTTCCGATCTNR
Anti-ILL: AGATCGGAAGAGC(InvdT)
Adaptor 2
3ILL-NR: CAGACGTGTGCTCTTCCGATCTNR
ILL-Lib1: AATGATACGGCGACCACCGA
ILL-Lib2: CAAGCAGAAGACGGCATACGA
ILL-HT1: AATGATACGGCGACCACCGAGATCTACACATGCATACACTCTTTCCCTACACGACGCTCTTCCGATCT
ILL-HT2:AATGATACGGCGACCACCGAGATCTACACCGTACGACACTCTTTCCCTACACGACGCTCTTCCGATCT
ILL-BC1: CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC