Continued the extractions that Steven began yesterday and this morning using the E.Z.N.A. Mollusc DNA Kit (Omega Bio-Tek) after the RNase treatment @ 70C.
- Samples were cooled to RT (~10mins)
- Added 300μL of 100% EtOH to each (equivalent to the volume of aqueous phase Steven recovered from each sample)
- Followed manufacturer’s protocol
- Eluted all samples with 50μL of Elution Buffer
- Spec’d on Roberts Lab NanoDrop1000
Results:
DNA looks absolutely pristine and has amazing yields.
Will check gDNA integrity via agarose gel tomorrow.
Data has been entered into the master spreadsheet for this project: Ostrea lurida Project Master Tissue Sample List