Last Friday, Ronit quantified 1:10 dilutions of the RNA I isolated on 20181003 and the RNA he finished isolating on 20181011, but two of the samples (D11-C, T10-C) were still too concentrated.

I made 1:20 dilutions (1uL RNA in 19uL 0.1% DEPC-treated H₂O) and quantified them using the Roberts Lab Qubit 3.0, with the RNA HS assay. Used 1uL of the diluted RNA.

RESULTS

Qubit data (Google Sheet):

• 20181016_qubit_Cgigas_RNA

Everything looks good. Added final concentration values (Qubit data x 20, to account for dilution factor) to Ronit’s master sheet (Google Sheet):

• Exposure 8/29-8/30 C.Gigas

Will proceed with DNasing.